rnase h treatment (New England Biolabs)

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New England Biolabs rnase h treatment
Rnase H Treatment, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 5330 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rnase+h+treatment/RNase+H/bio_rxiv__64898__2026__03__08__710212-282-27-30
Average 99 stars, based on 5330 article reviews

rnase h treatment - by Bioz Stars, 2026-09

99/100 stars

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Next-Generation Sequencing:

Article Title: Physiological roles of an Acinetobacter -specific σ factor.
Article Snippet: .. Briefly, DNA oligos complementary to A. baumannii 23S, 16S, and 5S rRNA were annealed to the total RNA samples, RNase H treatment was performed to cleave the annealed rRNA, and the DNA oligos were removed with Turbo DNase I. rRNA-depleted samples were prepared for next-generation sequencing using NEBNext Ultra II Directional RNA Library Prep kit (NEB; E7765S) and NEBNext Multiplex Oligos for Illumina (NEB; E6640S). .. Libraries were sequenced on Illumina NovaSeq 6000 2 × 150 with the UW-Madison Biotechnology Center at ~10 million reads per sample.

Article Title: Physiological roles of an Acinetobacter -specific σ factor
Article Snippet: .. Briefly, DNA oligos complementary to A. baumannii 23S, 16S, and 5S rRNA were annealed to the total RNA samples, RNase H treatment was performed to cleave the annealed rRNA, and the DNA oligos were removed with Turbo DNase I. rRNA-depleted samples were prepared for next-generation sequencing using NEBNext Ultra II Directional RNA Library Prep kit (NEB; E7765S) and NEBNext Multiplex Oligos for Illumina (NEB; E6640S). .. Libraries were sequenced on Illumina NovaSeq 6000 2 × 150 with the UW-Madison Biotechnology Center at ~10 million reads per sample.

Multiplex Assay:

Staining:

Article Title: Senataxin regulates cisplatin resistance through an R-loop-mediated mechanism in HPV-associated head and neck cancer
Article Snippet: .. Serial sections of tissue as A-B stained with S9.6 antibody at 1:1000 dilution with 25 units of RNase H treatment (NEB) overnight prior to blocking (C is a higher magnification of image D). ..

Blocking Assay:

Purification:

Article Title: RRTF1 promotes touch-responses in Arabidopsis shoots independent of jasmonic acid
Article Snippet: .. RNA samples were digested with DNase I (NEB) and purified using Monarch RNA Cleanup Kit (NEB). cDNA was synthesized using ProtoScript II Reverse Transcriptase (NEB), followed by RNase H treatment (NEB). .. QPCR reactions were run using PowerUp Sybr Green Master mix (Applied Biosystems) on a Bio-Rad CFX96 Real-Time PCR Detection system.

Synthesized:

Reverse Transcription:

Incubation:

Article Title: Supplementary Information for Excessive nucleic acid R-loops induce mitochondria-dependent epithelial cell necroptosis and drive spontaneous intestinal inflammation
Article Snippet: For S9.6 immunofluorescence analysis, NCM460 cells were fixed with ice-cold methanol for 10 min at -20°C and then incubated with 6 μg/ml RNase A (R1030, Solarbio) for 30 min at 37°C using 1× RNase A buffer (10 mM Tris-HCl, 5 mM EDTA, 5 mM NaCl). .. For RNase H treatment, fixed cells were also incubated with 7 μg/ml RNase H (M0297, NEB) for 4 h at 37°C using 1× RNase H buffer (75 mM KCl, 3 mM MgCl2, 50 mM Tris-HCl (pH 8.3), and 10 mM DTT). ..

Article Title: GPATCH4 functions as a regulator of nucleolar R-loops in hepatocellular carcinoma cells
Article Snippet: The R-loop signals were detected with ECL system by Amersham Imager 680. .. For RNase H treatment, 2 μg of genomic DNA was incubated with 5 U of RNase H (NEB, M0297L) in supplied reaction buffer overnight at 37°C. .. For RNase T1 treatment, 2 μg of genomic DNA was incubated with 1000 U of RNase T1 (Thermo Scientific, EN0541) in 50 mM Tris–HCl, pH 7.5, and 2 mM EDTA for 30 min at 37°C.

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